Process flow of lyophilized collagen powder

Lyophilized collagen powder is a sterile powder made by freezing the liquid into a solid-state in a sterile environment and drying the water by sublimation under a vacuum. The following is a detailed description of the lyophilized collagen powder process.

Here is the content list:

l Pretreatment

l Cooling and curing process

l Sublimation drying process

l Desorption drying process

l Encapsulation and storage

Pretreatment

Before freeze-drying, the frozen material, some necessary physical and chemical treatments must be carried out, including cleaning, grating, slicing, sterilization, concentration, etc. Before freeze-drying cosmetic products, some additives should be added to ensure the good effect of lyophilized collagen powder, maintain the biological activity of the frozen products, and better appearance of the structure. For additives, they can be broadly classified into several categories according to their functions: lyophilization protectants, emulsifiers, fillers, antioxidants, acid-base regulators, etc. According to the experimental findings in recent years, sugars are very effective lyophilization protectants as well as fillers, with different contents to make it freeze out in shape, tough and tangible.

Cooling and curing process

The cooling and curing process of the material is to cool the material components, not only to make the free water in the material completely freeze but also to make the other parts of the material completely solidify and form a solid non-crystal.

The cooling rate of collagen during the cooling and curing process is also important. Generally, rapid cooling can keep the material from dehydrating too much during the freezing process and achieve partial vitrification, but too fast cooling can also cause undesirable phenomena such as cell damage and material fracture. Therefore, the process of cooling and curing lyophilized collagen powder appears to be very important.

Sublimation drying process

For the analysis of the freeze-drying process, the water in collagen can be divided into two categories: one is the water that can be frozen into ice at low temperature, and this part of water can be called "free water"; the other is the water that cannot be frozen at low temperature, and this part of water can be regarded as being "bound ", called "bonded water". Primary drying, also known as sublimation drying, means heating collagen at low temperature to make the "free water" which is frozen into ice sublimate directly into water vapor.

Desorption drying process

Desorption drying, also known as secondary drying, is heated at a higher temperature, so that the material is adsorbed part of the "bound water" desorption, into the "free" liquid water, and then heat absorption evaporation into water vapor. At the end of the first stage of drying, the surface of the porous structure and polar groups of the dried material is still adsorbed with unfrozen bound water. Because of the high energy of adsorption, a high temperature and sufficient heat must be provided to achieve the desorption of bound water. The temperature should not be too high, otherwise, it will cause the collagen to overheat and denature.

Encapsulation and storage

After secondary drying, the lyophilized collagen powder is encapsulated and stored. Sealing must still be carried out under vacuum conditions, or filled with inert gas conditions. When freezing the material in Xilin bottles, the semi-corked state (the stopper should not be fully compacted), the channels on both sides of the rubber stopper can ensure that the "free water" can be evaporated into water vapor smoothly, which can be led from the drying chamber through the vacuum channel and sent to the vacuum chamber, or filled with inert gas to the bottle for protection, and finally the mechanical device for capping, out of the warehouse Then it is ready.

If you want to know more about lyophilized collagen powder, you can contact Guangdong Victory Biotech Co.