Functions for making three types of bone graft substitutes

There are three types of bone graft substitutes: those that can be used to promote new bone growth, those implants that can induce new bone formation and promote bone growth, and those that treat bone defects, induce new bone formation and promote bone growth. The steps for making these three collagen bone graft substitutes are described below.

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l Substitutes used to promote bone growth

l Substitutes used to Induce new bone formation

l Substitutes used to promote bone growth in

Substitutes used to promote bone growth

The steps for making autograft bone graft substitutes that can be used to promote new bone growth include.

(1) Combining the following components.

a) Autologous blood (ab), or autologous blood and platelet-rich plasma (prp), or platelet-rich plasma (prp), (selected from bmp-6, bmp-2, bmp-7, bmp-4, bmp-5, bmp-9, bmp-12, and bmp-13, their analogs, their heterodimers, and their combinations of bone-forming bone morphogenic proteins).

b) Coagulants (which are selected from ionic solutions or pharmacologically acceptable calcium salts in nanoparticles and ca++ microsphere couples).

c) Compression-resistant matrix (crm).

(2) Incubation of the composition from the previous step for some time sufficient to form a gel of condensate.

Substitutes used to Induce new bone formation

In another preparation, other ingredients need to be added before step (2). The steps of the autologous bone graft substitute composition for treating bone defects, inducing new bone formation, and promoting bone growth thereof include.

(1) Combining the following components.

a) Autologous blood.

b) A bone-forming bone morphogenetic

c) Coagulants, selected from: ionic solutions or pharmacologically acceptable calcium salts in nanoparticles and ca++ microsphere couples.

d) A compression-resistant matrix (crm).

The compression-resistant substrate can be chosen arbitrarily. Exogenously provided in vitro expanded autologous or allogeneic mesenchymal cell populations from bone marrow, adipose tissue, periosteal layer, and umbilical cord.

(2) Incubate the composition from the previous step for some time sufficient to form a gel of condensate.

Substitutes used to promote bone growth in

The steps of the autologous collagen bone graft substitute block composition prepared for use in treating bone defects, inducing new bone formation, and promoting bone growth are.

(1) Combining the following components.

a) Autologous blood.

b)osteogenic bone morphogenetic protein.

c) A coagulant, selected from an ionic solution or a pharmacologically acceptable calcium salt in a coupling of nanoparticles and ca++ microspheres.

d) Compression-resistant matrix (crm).

(e) Reversed-phase heat-sensitive bioresorbable polymers.

The compression-tolerant matrix can be chosen arbitrarily. Exogenously provided in vitro expanded autologous or allogeneic mesenchymal cell populations from bone marrow, adipose tissue, periosteal layer, and/or umbilical cord. A demineralized bone matrix can also be chosen arbitrarily.

(2) The composition of collagen bone graft substitute block in the previous step of the incubation step lasts for some time sufficient to form a gel of condensate. Said condensate gel has rheology. In this way, it can be injected into the liquid phase at room temperature. And, the coagulant gel forms a biocompatible gel at the delivery site/implantation site when the temperature is raised to body temperature (37°C).

Preferably, the osteogenic bmp used in the abgs compositions described herein is selected from: bmp-2, bmp-4, bmp-5, bmp-6, bmp-7, bmp-8, bmp-9, bmp-12, and bmp-13, analogs thereof, heterodimers thereof, and combinations thereof. More preferably, said osteogenic bmp is bmp-6.

These collagen bone graft substitutes are capable of increasing the bone mineral density of the femoral head and vertebral bodies in severely osteoporotic patients suffering from postmenopausal osteoporosis or advanced osteoporosis or steroid-induced osteoporosis. These patients with severe osteoporosis may experience hip and vertebral fractures following a fall.